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Quantitative Human MDH2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX402)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human MDH2 (glycosylated) sandwich ELISA kit is designed to detect human malate dehydrogenase 2 (MDH2) levels. MDH2 is an enzyme located in the mitochondria, which plays a critical role in the citric acid cycle. MDH2 catalyzes the conversion of malate to oxaloacetate, a key step in energy production within cells. This enzyme is essential for cellular respiration and overall energy metabolism. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.302 ng/mL, which can accurately detect low concentrations of MDH2 in the sample.
Target MDH2
O-Glycosylation Site 33
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.302 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1.50 ng/mL-45 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Malate dehydrogenase 2
Alternate Names MDH2; Malate dehydrogenase 2; MDH; MOR1
Uniprot No. P40926
Application The quantitative human MDH2 (glycosylated) sandwich ELISA kit is designed for the quantification of glycosylated MDH2 in samples. This measurement is relevant in studies of cellular metabolism, mitochondrial functions, and diseases associated with metabolic dysfunctions. The kit can be used to investigate conditions such as cancer, neurodegenerative disorders, and metabolic syndromes.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-105%;
Plasma sample: n=4, 95-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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