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Quantitative Human MDH2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX402

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Specifications
Product Description
The quantitative human MDH2 (glycosylated) sandwich ELISA kit is designed to detect human malate dehydrogenase 2 (MDH2) levels. MDH2 is an enzyme located in the mitochondria, which plays a critical role in the citric acid cycle. MDH2 catalyzes the conversion of malate to oxaloacetate, a key step in energy production within cells. This enzyme is essential for cellular respiration and overall energy metabolism. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.302 ng/mL, which can accurately detect low concentrations of MDH2 in the sample.
Target
MDH2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.302 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.50 ng/mL-45 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Malate dehydrogenase 2
Alternate Names
MDH2; Malate dehydrogenase 2; MDH; MOR1
Uniprot No.
O-Glycosylation Site
33
Application Notes
Application
The quantitative human MDH2 (glycosylated) sandwich ELISA kit is designed for the quantification of glycosylated MDH2 in samples. This measurement is relevant in studies of cellular metabolism, mitochondrial functions, and diseases associated with metabolic dysfunctions. The kit can be used to investigate conditions such as cancer, neurodegenerative disorders, and metabolic syndromes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-105%;
Plasma sample: n=4, 95-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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