Quantitative Human MHCB (Glycosylated) Sandwich ELISA Kit

The quantitative human MHCB (glycosylated) sandwich ELISA kit is designed to detect human major histocompatibility complex class 1B (MHCB) levels. MHCB refers to a group of non-classical MHC class I molecules involved in immune regulation and antigen presentation. These molecules often exhibit specialized functions beyond traditional antigen presentation to T cells. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.118 ng/mL, which can accurately detect low concentrations of MHCB in the sample.

MHCB

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.118 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Major histocompatibility complex class 1B

MHCB; Major histocompatibility complex class 1B; HLAB; SPDA1; Leukocyte antigen B

110

The quantitative human MHCB (glycosylated) sandwich ELISA kit is utilized in research investigating immune responses, transplantation, and autoimmune diseases, where the specific roles of non-classical MHC molecules are explored. Researchers employ this ELISA to quantify MHCB levels, providing insights into their contributions to immune modulation and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.