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Quantitative Human MHCB (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX146)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human MHCB (glycosylated) sandwich ELISA kit is designed to detect human major histocompatibility complex class 1B (MHCB) levels. MHCB refers to a group of non-classical MHC class I molecules involved in immune regulation and antigen presentation. These molecules often exhibit specialized functions beyond traditional antigen presentation to T cells. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.118 ng/mL, which can accurately detect low concentrations of MHCB in the sample.
Target MHCB
N-Glycosylation Site 110
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.118 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Major histocompatibility complex class 1B
Alternate Names MHCB; Major histocompatibility complex class 1B; HLAB; SPDA1; Leukocyte antigen B
Uniprot No. P01889
Application The quantitative human MHCB (glycosylated) sandwich ELISA kit is utilized in research investigating immune responses, transplantation, and autoimmune diseases, where the specific roles of non-classical MHC molecules are explored. Researchers employ this ELISA to quantify MHCB levels, providing insights into their contributions to immune modulation and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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