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Quantitative Human NFASC (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX103)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NFASC (glycosylated) sandwich ELISA kit is designed to detect human neurofascin (NFASC) levels. NFASC is a cell adhesion molecule belonging to the L1 family, playing a crucial role in axonal guidance, neuronal migration, and the formation of nodes of Ranvier in myelinated axons. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.129 ng/mL, which can accurately detect low concentrations of NFASC in the sample.
Target NFASC
N-Glycosylation Site 305, 409, 446, 483, 752, 778, 973, 988
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.129 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-20 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Neurofascin
Alternate Names NFASC; Neurofascin; NF
Uniprot No. O94856
Application The quantitative human NFASC (glycosylated) sandwich ELISA kit is valuable for research investigating neurological disorders, such as multiple sclerosis, schizophrenia, and autism spectrum disorders, where NFASC plays a significant role in neuronal connectivity and myelination. The quantitative data obtained from this kit allows for precise analysis of NFASC levels, contributing to a better understanding of its involvement in these complex neurological conditions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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