Quantitative Human NFASC (Glycosylated) Sandwich ELISA Kit

The quantitative human NFASC (glycosylated) sandwich ELISA kit is designed to detect human neurofascin (NFASC) levels. NFASC is a cell adhesion molecule belonging to the L1 family, playing a crucial role in axonal guidance, neuronal migration, and the formation of nodes of Ranvier in myelinated axons. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.129 ng/mL, which can accurately detect low concentrations of NFASC in the sample.

NFASC

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.129 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-20 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Neurofascin

NFASC; Neurofascin; NF

305, 409, 446, 483, 752, 778, 973, 988

The quantitative human NFASC (glycosylated) sandwich ELISA kit is valuable for research investigating neurological disorders, such as multiple sclerosis, schizophrenia, and autism spectrum disorders, where NFASC plays a significant role in neuronal connectivity and myelination. The quantitative data obtained from this kit allows for precise analysis of NFASC levels, contributing to a better understanding of its involvement in these complex neurological conditions.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.