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Quantitative Human NTRK2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX615

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Specifications
Product Description
The quantitative human NTRK2 (glycosylated) sandwich ELISA kit is designed to detect human neurotrophic tyrosine kinase receptor type 2 (NTRK2) levels. NTRK2 is a receptor tyrosine kinase that binds brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5). This receptor plays a critical role in the development, survival, and functions of neurons. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.064 ng/mL, which can accurately detect low concentrations of NTRK2 in the sample.
Target
NTRK2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.064 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Neurotrophic tyrosine kinase receptor type 2
Alternate Names
NTRK2; Neurotrophic tyrosine kinase receptor type 2; TRK-B; Tropomyosin-related kinase B
Uniprot No.
N-Glycosylation Site
67, 95, 121, 178, 205, 241, 254, 280, 325, 338, 412
Application Notes
Application
The quantitative human NTRK2 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated NTRK2 levels in biological samples. The kit is applicable in studies of neuroscience, neurotrophin signaling, and synaptic plasticity. It is useful in fields such as neurobiology and pharmacology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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