Quantitative Human P4Hα3 (Glycosylated) Sandwich ELISA Kit

The quantitative human P4Hα3 (glycosylated) sandwich ELISA kit is designed to detect human prolyl-4-hydroxylase α polypeptide 3 (P4Hα3) levels. P4Hα3 is a subunit of prolyl 4-hydroxylase (P4H), an enzyme that catalyzes the formation of 4-hydroxyproline in collagen. This hydroxylation is essential for the proper folding and stability of collagen, a major component of the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.095 ng/mL, which can accurately detect low concentrations of P4Hα3 in the sample.

P4Hα3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.095 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Prolyl-4-hydroxylase α polypeptide 3

P4Hα3; Prolyl-4-hydroxylase α polypeptide 3; Procollagen-proline; Alpha polypeptide III

242, 482

The quantitative human P4Hα3 (glycosylated) sandwich ELISA kit is used to assess P4Hα3 levels in biological samples. It is applicable in studies related to collagen biosynthesis, extracellular matrix biology, and connective tissue disorders. The kit is valuable in fields such as cell biology and biochemistry.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.