0
Inquiry Basket

There is no product in the shopping cart, buy it!

Quantitative Human P4Hα3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX666)

Datasheet
SizeQtyAdd To Basket
1×48 T
1×96 T

Product Description The quantitative human P4Hα3 (glycosylated) sandwich ELISA kit is designed to detect human prolyl-4-hydroxylase α polypeptide 3 (P4Hα3) levels. P4Hα3 is a subunit of prolyl 4-hydroxylase (P4H), an enzyme that catalyzes the formation of 4-hydroxyproline in collagen. This hydroxylation is essential for the proper folding and stability of collagen, a major component of the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.095 ng/mL, which can accurately detect low concentrations of P4Hα3 in the sample.
Target P4Hα3
N-Glycosylation Site 242, 482
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.095 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Prolyl-4-hydroxylase α polypeptide 3
Alternate Names P4Hα3; Prolyl-4-hydroxylase α polypeptide 3; Procollagen-proline; Alpha polypeptide III
Uniprot No. Q7Z4N8
Application The quantitative human P4Hα3 (glycosylated) sandwich ELISA kit is used to assess P4Hα3 levels in biological samples. It is applicable in studies related to collagen biosynthesis, extracellular matrix biology, and connective tissue disorders. The kit is valuable in fields such as cell biology and biochemistry.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
Online Inquiry
Creative Biolabs-Glycoprotein Follow us on
  • ()
    ()
    ()
ISO 9001 Certified - Creative Biolabs Quality Management System.