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Quantitative Human PNPLA2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX727

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Specifications
Product Description
The quantitative human PNPLA2 (glycosylated) sandwich ELISA kit is designed to detect human patatin like phospholipase domain containing protein 2 (PNPLA2) levels. PNPLA2 is an enzyme, which is also known as adipose triglyceride lipase (ATGL). PNPLA2 catalyzes the hydrolysis of triglycerides. This process releases fatty acids. It plays a role in lipid metabolism. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.142 ng/mL, which can accurately detect low concentrations of PNPLA2 in the sample.
Target
PNPLA2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.142 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Patatin like phospholipase domain containing protein 2
Alternate Names
PNPLA2; Patatin like phospholipase domain containing protein 2; ATGL; TTS2; Transport-secretion protein 2
Uniprot No.
N-Glycosylation Site
39
Application Notes
Application
The quantitative human PNPLA2 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PNPLA2 in samples. This quantification is important for research on lipid metabolism and enzyme activity. The kit also has potential applications in investigations of obesity.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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