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Quantitative Human PTPRF (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX218)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PTPRF (glycosylated) sandwich ELISA kit is designed to detect human protein tyrosine phosphatase receptor type F (PTPRF) levels. PTPRF is a transmembrane protein tyrosine phosphatase involved in cell growth regulation, adhesion, and signaling pathways. It plays a role in various cellular processes, including tumorigenesis and neuronal development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.358 ng/mL, which can accurately detect low concentrations of PTPRF in the sample.
Target PTPRF
N-Glycosylation Site 117, 250, 295, 721, 966
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.358 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1.25 ng/mL-50 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Protein tyrosine phosphatase receptor type F
Alternate Names PTPRF; Protein tyrosine phosphatase receptor type F
Uniprot No. P10586
Application The quantitative human PTPRF (glycosylated) sandwich ELISA kit is valuable in research investigating cancer biology, neurological disorders, and cell signaling pathways. Researchers utilize this ELISA to quantify PTPRF levels, providing insights into its role in these conditions and potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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