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Quantitative Human PTPRF (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX218

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Specifications
Product Description
The quantitative human PTPRF (glycosylated) sandwich ELISA kit is designed to detect human protein tyrosine phosphatase receptor type F (PTPRF) levels. PTPRF is a transmembrane protein tyrosine phosphatase involved in cell growth regulation, adhesion, and signaling pathways. It plays a role in various cellular processes, including tumorigenesis and neuronal development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.358 ng/mL, which can accurately detect low concentrations of PTPRF in the sample.
Target
PTPRF
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.358 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.25 ng/mL-50 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Protein tyrosine phosphatase receptor type F
Alternate Names
PTPRF; Protein tyrosine phosphatase receptor type F
Uniprot No.
N-Glycosylation Site
117, 250, 295, 721, 966
Application Notes
Application
The quantitative human PTPRF (glycosylated) sandwich ELISA kit is valuable in research investigating cancer biology, neurological disorders, and cell signaling pathways. Researchers utilize this ELISA to quantify PTPRF levels, providing insights into its role in these conditions and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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