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Quantitative Human RBP3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX221)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human RBP3 (glycosylated) sandwich ELISA kit is designed to detect human retinol binding protein 3 (RBP3) levels. RBP3, also known as interstitial retinol-binding protein (IRBP), is a protein involved in retinol transport in the eye's interphotoreceptor matrix, essential for the visual cycle. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.062 ng/mL, which can accurately detect low concentrations of RBP3 in the sample.
Target RBP3
N-Glycosylation Site 205, 515
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.062 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Retinol binding protein 3
Alternate Names RBP3; Retinol binding protein 3; RBP-3; IRBP; Interphotoreceptor retinoid-binding protein
Uniprot No. P10745
Application The quantitative human RBP3 (glycosylated) sandwich ELISA kit is valuable in research investigating retinal diseases, such as retinitis pigmentosa and age-related macular degeneration, and in studies of vitamin A metabolism in the eye. Researchers utilize this ELISA to quantify RBP3 levels, providing insights into retinal health and potential diagnostic or prognostic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 95-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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