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Quantitative Human RIPK1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX571)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human RIPK1 (glycosylated) sandwich ELISA kit is designed to detect human receptor interacting serine threonine kinase 1 (RIPK1) levels. RIPK1 is a protein kinase, which plays a complex role in cell signaling. RIPK1 can promote both cell survival and cell death, depending on the context. It is involved in inflammation, apoptosis, and necroptosis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.091 ng/mL, which can accurately detect low concentrations of RIPK1 in the sample.
Target RIPK1
N-Glycosylation Site 603
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.091 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Receptor interacting serine threonine kinase 1
Alternate Names RIPK1; Receptor interacting serine threonine kinase 1; RIP; Receptor-interacting protein; Cell death protein RIP
Uniprot No. Q13546
Application The quantitative human RIPK1 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and immunology, particularly in studies of cell death. Researchers can measure RIPK1 levels in cells and tissues. Applications include studies of inflammation, cancer, and neurodegeneration.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-90%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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