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Quantitative Human SIGLEC15 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX654

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Specifications
Product Description
The quantitative human SIGLEC15 (glycosylated) sandwich ELISA kit is designed to detect human sialic acid binding ig like lectin 15 (SIGLEC15) levels. SIGLEC15 is a protein expressed on various immune cells, including macrophages and dendritic cells. It belongs to the SIGLEC family, which is involved in recognizing sialic acids on cell surfaces. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.087 ng/mL, which can accurately detect low concentrations of SIGLEC15 in the sample.
Target
SIGLEC15
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
0.087 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sialic acid binding ig like lectin 15
Alternate Names
SIGLEC15; Sialic acid binding ig like lectin 15; CD33L3
Uniprot No.
N-Glycosylation Site
172
Application Notes
Application
The quantitative human SIGLEC15 (glycosylated) sandwich ELISA kit is used to determine SIGLEC15 concentrations in biological samples. It proves valuable in research investigating immune regulation, cell-cell interactions, and the role of SIGLEC15 in immune-related diseases. This kit finds application in fields such as immunology, cell biology, and potentially in studies related to cancer immunotherapy.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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