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Quantitative Human SPN (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX257

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Specifications
Product Description
The quantitative human SPN (glycosylated) sandwich ELISA kit is designed to detect human sialophorin (SPN) levels. SPN is a protein that is found on the surface of leukocytes. This protein plays a role in cell adhesion and migration. It is involved in immune responses and cancer metastasis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.083 ng/mL, which can accurately detect low concentrations of SPN in the sample.
Target
SPN
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.083 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sialophorin
Alternate Names
SPN; Sialophorin; GALGP, LSN
Uniprot No.
N-Glycosylation Site
239
O-Glycosylation Site
21, 22, 26, 28, 29, 35, 36, 37, 41, 42, 46, 47, 48, 50, 58, 69, 99, 103, 109, 113, 114, 136, 137, 173, 178
Application Notes
Application
The quantitative human SPN (glycosylated) sandwich ELISA kit enables researchers to measure SPN levels in biological samples. It can be used in studies examining immune cell functions, inflammation, and cancer. The kit is applicable in immunological research and the development of new diagnostic tools.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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