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Quantitative Human SPN (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX257)

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1×48 T
1×96 T

Product Description The quantitative human SPN (glycosylated) sandwich ELISA kit is designed to detect human sialophorin (SPN) levels. SPN is a protein that is found on the surface of leukocytes. This protein plays a role in cell adhesion and migration. It is involved in immune responses and cancer metastasis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.083 ng/mL, which can accurately detect low concentrations of SPN in the sample.
Target SPN
N-Glycosylation Site 239
O-Glycosylation Site 21, 22, 26, 28, 29, 35, 36, 37, 41, 42, 46, 47, 48, 50, 58, 69, 99, 103, 109, 113, 114, 136, 137, 173, 178
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.083 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Sialophorin
Alternate Names SPN; Sialophorin; GALGP, LSN
Uniprot No. P16150
Application The quantitative human SPN (glycosylated) sandwich ELISA kit enables researchers to measure SPN levels in biological samples. It can be used in studies examining immune cell functions, inflammation, and cancer. The kit is applicable in immunological research and the development of new diagnostic tools.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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