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Quantitative Human TLR3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX48

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Specifications
Product Description
The quantitative human TLR3 (glycosylated) sandwich ELISA kit is designed to detect human toll like receptor 3 (TLR3) levels. TLR3 is a transmembrane protein that plays a crucial role in the innate immune system by recognizing double-stranded RNA, a molecular pattern associated with viral infection. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.117 ng/mL, which can accurately detect low concentrations of TLR3 in the sample.
Target
TLR3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.117 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Toll like receptor 3
Alternate Names
TLR3; Toll like receptor 3; CD283
Uniprot No.
N-Glycosylation Site
52, 57, 70, 124, 196, 247, 252, 265, 275, 291, 398, 413, 507, 636, 662
Application Notes
Application
The quantitative human TLR3 (glycosylated) sandwich ELISA kit utilizes a sandwich ELISA format, allowing researchers to quantify TLR3 expression, which is essential for studying immune responses to viral infections, as well as the role of TLR3 in inflammatory and autoimmune diseases. This assay is a valuable tool for investigating TLR3-mediated signaling pathways in various research contexts.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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