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Quantitative Human TLR4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX9)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human TLR4 (glycosylated) sandwich ELISA kit is designed to detect human toll like receptor 4 (TLR4) levels. TLR4 is a transmembrane protein that plays a crucial role in the innate immune system by recognizing lipopolysaccharide (LPS) and initiating inflammatory responses. It is a key component in the detection of bacterial pathogens. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.245 ng/mL, which can accurately detect low concentrations of TLR4 in the sample.
Target TLR4
N-Glycosylation Site 35, 173, 205, 282, 309, 497, 526, 575, 624, 630
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.245 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-20 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Toll like receptor 4
Alternate Names TLR4; Toll like receptor 4; CD284; TOLL
Uniprot No. O00206
Application The quantitative human TLR4 (glycosylated) sandwich ELISA kit is valuable for researchers investigating the role of TLR4 in inflammatory diseases, sepsis, and other conditions involving immune activation. It allows for the precise quantification of TLR4 levels, aiding in the understanding of TLR4 signaling pathways and potential therapeutic interventions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-105%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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