Quantitative Human TLR4 (Glycosylated) Sandwich ELISA Kit

The quantitative human TLR4 (glycosylated) sandwich ELISA kit is designed to detect human toll like receptor 4 (TLR4) levels. TLR4 is a transmembrane protein that plays a crucial role in the innate immune system by recognizing lipopolysaccharide (LPS) and initiating inflammatory responses. It is a key component in the detection of bacterial pathogens. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.245 ng/mL, which can accurately detect low concentrations of TLR4 in the sample.

TLR4

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.245 ng/mL

Quantitative sandwich ELISA

1 ng/mL-20 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Toll like receptor 4

TLR4; Toll like receptor 4; CD284; TOLL

35, 173, 205, 282, 309, 497, 526, 575, 624, 630

The quantitative human TLR4 (glycosylated) sandwich ELISA kit is valuable for researchers investigating the role of TLR4 in inflammatory diseases, sepsis, and other conditions involving immune activation. It allows for the precise quantification of TLR4 levels, aiding in the understanding of TLR4 signaling pathways and potential therapeutic interventions.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-105%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.