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Quantitative Human UPK2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX17)

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1×48 T
1×96 T

Product Description The quantitative human UPK2 (glycosylated) sandwich ELISA kit is designed to detect human uroplakin 2 (UPK2) levels. UPK2 is a major component of the asymmetric unit membrane (AUM) found in urothelial cells, playing a crucial role in the barrier function of the bladder epithelium. It is essential for maintaining the integrity and impermeability of the urinary tract. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of UPK2 in the sample.
Target UPK2
N-Glycosylation Site 28, 57, 66
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Uroplakin 2
Alternate Names UPK2; Uroplakin 2; UPII; Uroplakin II
Uniprot No. O00526
Application The quantitative human UPK2 (glycosylated) sandwich ELISA kit is valuable for researchers investigating bladder cancer, interstitial cystitis, and other urothelial disorders, where UPK2 expression is often altered. By quantifying UPK2 levels, researchers can gain insights into the role of this protein in disease pathogenesis and explore potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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