Quantitative Human UPK2 (Glycosylated) Sandwich ELISA Kit

The quantitative human UPK2 (glycosylated) sandwich ELISA kit is designed to detect human uroplakin 2 (UPK2) levels. UPK2 is a major component of the asymmetric unit membrane (AUM) found in urothelial cells, playing a crucial role in the barrier function of the bladder epithelium. It is essential for maintaining the integrity and impermeability of the urinary tract. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of UPK2 in the sample.

UPK2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Uroplakin 2

UPK2; Uroplakin 2; UPII; Uroplakin II

28, 57, 66

The quantitative human UPK2 (glycosylated) sandwich ELISA kit is valuable for researchers investigating bladder cancer, interstitial cystitis, and other urothelial disorders, where UPK2 expression is often altered. By quantifying UPK2 levels, researchers can gain insights into the role of this protein in disease pathogenesis and explore potential diagnostic or therapeutic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.