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Quantitative Human WNT11 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX128

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Specifications
Product Description
The quantitative human WNT11 (glycosylated) sandwich ELISA kit is designed to detect human wingless type MMTV integration site family, member 11 (WNT11) levels. WNT11 is a secreted glycoprotein that plays a critical role in various developmental processes, including cell fate determination and tissue polarity, through the Wnt signaling pathway. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.126 ng/mL, which can accurately detect low concentrations of WNT11 in the sample.
Target
WNT11
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.126 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Wingless type MMTV integration site family, member 11
Alternate Names
WNT11; Wingless type MMTV integration site family, member 11; WNT11b
Uniprot No.
N-Glycosylation Site
40, 90, 160, 300, 304
Application Notes
Application
The quantitative human WNT11 (glycosylated) sandwich ELISA kit is valuable for researchers investigating the role of WNT11 in development, disease, and tissue regeneration. Scientists employ this ELISA to quantify WNT11 levels, providing insights into its involvement in conditions like cancer, fibrosis, and developmental disorders.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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