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Quantitative Human WNT11 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX128)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human WNT11 (glycosylated) sandwich ELISA kit is designed to detect human wingless type MMTV integration site family, member 11 (WNT11) levels. WNT11 is a secreted glycoprotein that plays a critical role in various developmental processes, including cell fate determination and tissue polarity, through the Wnt signaling pathway. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.126 ng/mL, which can accurately detect low concentrations of WNT11 in the sample.
Target WNT11
N-Glycosylation Site 40, 90, 160, 300, 304
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.126 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Wingless type MMTV integration site family, member 11
Alternate Names WNT11; Wingless type MMTV integration site family, member 11; WNT11b
Uniprot No. O96014
Application The quantitative human WNT11 (glycosylated) sandwich ELISA kit is valuable for researchers investigating the role of WNT11 in development, disease, and tissue regeneration. Scientists employ this ELISA to quantify WNT11 levels, providing insights into its involvement in conditions like cancer, fibrosis, and developmental disorders.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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