GlycoErase™ G6PC1 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF393)

Description	GlycoErase™ G6PC1 knockout HEK293 cell line is a human embryonic kidney (HEK293) model in which G6PC1 expression has been eliminated. G6PC1 encodes the catalytic subunit of glucose-6-phosphatase, a critical enzyme for glucose homeostasis that hydrolyzes glucose-6-phosphate to glucose and phosphate in gluconeogenesis and glycogenolysis. Mutations in G6PC1 cause glycogen storage disease type I (von Gierke disease), characterized by hypoglycemia and abnormal glycogen accumulation. The knockout of G6PC1 in the HEK293 background provides a human-relevant system for investigating glucose regulation, metabolic control, and glycogen storage disorders, while supporting translational research and therapeutic development in metabolic and genetic diseases.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	G6PC1
Full Name	Glucose-6-Phosphatase Catalytic Subunit 1
Alternative Name	G6PC; G6PT; GSD1; GSD1a; G6Pase
Location	17q21.31
Gene ID	2538
Summary	Glucose-6-phosphatase (G6PC) is an ER membrane enzyme that hydrolyzes glucose-6-phosphate to glucose and phosphate, playing a key role in gluconeogenesis and glycogenolysis. Mutations cause glycogen storage disease type I (von Gierke disease).