GlycoErase™ G6PC1 Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF277)

Description	GlycoErase™ G6PC1 knockout CHO-K1 cell line is a Chinese hamster ovary (CHO-K1) model in which G6PC1 expression has been eliminated. G6PC1 encodes the catalytic subunit of glucose-6-phosphatase, a key enzyme for glucose homeostasis that hydrolyzes glucose-6-phosphate to glucose and phosphate in gluconeogenesis and glycogenolysis. Mutations in G6PC1 are associated with glycogen storage disease type I (von Gierke disease), characterized by hypoglycemia and abnormal glycogen accumulation. The knockout of G6PC1 in the CHO-K1 background provides a specialized system for investigating glucose regulation, metabolic control, and glycogen storage disorders, while supporting drug development in metabolic and genetic diseases.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	G6PC1
Full Name	Glucose-6-Phosphatase Catalytic Subunit 1
Alternative Name	G6PC; G6PT; GSD1; GSD1a; G6Pase
Location	17q21.31
Gene ID	2538
Summary	Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.