GlycoErase™ HK1 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF410)

Description	GlycoErase™ HK1 knockout HEK293 cell line is a human embryonic kidney (HEK293) model in which HK1 expression has been deleted. HK1 encodes a ubiquitous hexokinase localized to the outer mitochondrial membrane, phosphorylating glucose in the first step of glycolysis. Mutations are linked to hemolytic anemia due to hexokinase deficiency. This knockout model supports investigations of glycolytic flux, mitochondrial glucose handling, and red blood cell metabolism, with applications in hematology, energy metabolism, and rare disease research.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	HK1
Full Name	Hexokinase 1
Alternative Name	HK; HKD; HKI; HXK1; NMSR; RP79; HMSNR; CNSHA5; HK1-ta; HK1-tb; HK1-tc; NEDVIBA; hexokinase
Location	10q22.1
Gene ID	3098
Summary	This hexokinase gene encodes a form of the enzyme that localizes to the mitochondrial outer membrane and is involved in glucose metabolism. Mutations are linked to hemolytic anemia. Alternative splicing produces tissue-specific isoforms.