GlycoErase™ PCK1 Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF538)

Description	GlycoErase™ PCK1 knockout HEK293T cell line is a HEK293T model deficient in PCK1 expression. PCK1 encodes cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), a key enzyme of gluconeogenesis regulated by hormones including insulin, glucagon, and glucocorticoids. Mutations result in PEPCK deficiency. This knockout enables mechanistic studies of gluconeogenic regulation, endocrine control of metabolism, and metabolic disease pathways in a human cell system.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PCK1
Full Name	Phosphoenolpyruvate Carboxykinase 1
Alternative Name	PCKDC; PEPCK1; PEPCKC; PEPCK-C
Location	20q13.31
Gene ID	5105
Summary	This gene encodes an enzyme critical for gluconeogenesis, converting oxaloacetate to phosphoenolpyruvate. It is regulated by insulin, glucocorticoids, and diet. Defects result in phosphoenolpyruvate carboxykinase deficiency.