GlycoErase™ PDK2 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF657)

Description	GlycoErase™ PDK2 knockout HepG2 cell line removes PDK2, a pyruvate dehydrogenase kinase that phosphorylates and inactivates the PDH complex, reducing mitochondrial glucose oxidation. Overexpression is linked to cancer and diabetes. This knockout provides a platform for studying PDH regulation, hepatic energy metabolism, and mechanisms of metabolic disease.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PDK2
Full Name	Pyruvate Dehydrogenase Kinase 2
Alternative Name	PDHK2; PDKII
Location	17q21.33
Gene ID	5164
Summary	This gene encodes a pyruvate dehydrogenase kinase that phosphorylates and inhibits the mitochondrial pyruvate dehydrogenase complex, downregulating glucose oxidation. Its overexpression has been linked to cancer and diabetes. Alternative splicing generates multiple isoforms.