GlycoErase™ PFKM Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF429)

Description	GlycoErase™ PFKM knockout HEK293 cell line is a HEK293 model without PFKM expression. PFKM encodes the muscle isoform of phosphofructokinase, catalyzing the rate-limiting step in glycolysis. Mutations cause glycogen storage disease type VII (Tarui disease). This knockout model enables human-focused studies of glycolytic regulation, metabolic diseases, and tumor glycolysis under altered energy demand.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PFKM
Full Name	Phosphofructokinase, Muscle
Alternative Name	GSD7; PFK1; PFKA; PFKX; PFK-1; PFK-A; ATP-PFK; PPP1R122
Location	12q13.11
Gene ID	5213
Summary	Phosphofructokinase (PFK) is a glycolytic enzyme with three isozymes in humans: muscle, liver, and platelet. The muscle isozyme is encoded by this gene, and mutations cause glycogen storage disease type VII (Tarui disease). Alternative splicing results in multiple isoforms.