GlycoFlux™ Human PFKM Overexpression Raji Cell Line (CAT#: GLJF-0925-JF958)

Description	GlycoFlux™ human PFKM overexpression Raji cell line is a stable cell line engineered to overexpress PFKM, the muscle isoform of phosphofructokinase that assembles into tissue-specific tetramers to catalyze the F6P→F1,6BP rate-setting step of glycolysis. Mutations cause glycogen storage disease VII (Tarui disease); this model enables investigation of allosteric regulation, subunit composition, and myopathic glycolysis defects.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Lymphoblast-like, suspension growth
Passage Ratio	1:2~1:4
Cell Line	Raji
Primary Disease	Burkitt's lymphoma
Lineage	B lymphocyte (B cell)
Lineage Subtype	Mature B cell, widely used in EBV-related studies

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & RPMI
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PFKM
Full Name	Phosphofructokinase, Muscle
Alternative Name	GSD7; PFK1; PFKA; PFKX; PFK-1; PFK-A; ATP-PFK; PPP1R122
Location	12q13.11
Gene ID	5213
Summary	Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.