GlycoFlux™ Human AKT3 Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF609)

Description	GlycoFlux™ human AKT3 overexpression HepG2 cell line is a stable cell line overexpressing AKT3, a serine/threonine kinase activated by growth factors such as insulin, IGF1, and PDGF. AKT3 regulates survival, proliferation, glycogen synthesis, glucose uptake, and tumorigenesis. This model provides a versatile platform for studies in cancer biology, insulin signaling, neuronal function, and targeted drug discovery.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AKT3
Full Name	Akt Serine/Threonine Kinase 3
Alternative Name	MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Location	1q43-q44
Gene ID	10000
Summary	This AKT kinase family member regulates signaling downstream of PDGF, insulin, and IGF1, affecting proliferation, survival, glycogen formation, and glucose uptake. Distinct isoforms arise from alternative splicing.