GlycoFlux™ Human GYG1 Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF638)

Description	GlycoFlux™ human GYG1 overexpression HepG2 cell line is a stable glycoengineered line that constitutively overexpresses glycogenin-1, the self-glucosylating primer for glycogen biosynthesis. By increasing primer abundance, it adjusts glycogen initiation to test consequences for donor-sugar generation, ER load tolerance, and glycan capping on hepatocyte receptors.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GYG1
Full Name	Glycogenin 1
Alternative Name	GYG; GSD15
Location	3q24
Gene ID	2992
Summary	This gene encodes glycogenin, a glycosyltransferase that self-primes glycogen synthesis by forming short glucose chains, which are elongated by glycogen synthase and branching enzyme. Mutations cause glycogen storage disease XV.