GlycoFlux™ Human PPP1R12B Overexpression HEK293T Cell Line (CAT#: GLJF-0925-JF561)

Description	GlycoFlux™ human PPP1R12B overexpression HEK293T cell line is a stable cell line designed to overexpress PPP1R12B, a myosin phosphatase regulatory subunit (MYPT2) found in heart, skeletal muscle, and brain. Together with PP1c-δ and sm-M20, MYPT2 forms the holoenzyme complex regulating myosin light chain dephosphorylation. This model enables studies on muscle-specific phosphatase regulation, cardiac physiology, and metabolic adaptations.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R12B
Full Name	Protein Phosphatase 1 Regulatory Subunit 12B
Alternative Name	MYPT2; PP1bp55
Location	1q32.1
Gene ID	4660
Summary	Myosin phosphatase consists of three subunits: PP1c-delta, MYPT, and sm-M20. MYPT1 is widely expressed, while MYPT2 is tissue-specific to the heart, skeletal muscle, and brain.