GlycoFlux™ Human RBCK1 Overexpression HEK293 Cell Line (CAT#: GLJF-0925-JF481)

Description	GlycoFlux™ human RBCK1 overexpression HEK293 cell line is a stable cell line designed to overexpress RBCK1, a multifunctional ubiquitin-binding protein with roles in transcriptional coactivation and NF-κB signaling. As part of the LUBAC ubiquitin ligase complex, RBCK1 contributes to linear ubiquitination and immune pathway activation, while mutations are implicated in glycogen storage disorders. This engineered model enables research into ubiquitination, inflammation, and links between protein quality control and carbohydrate metabolism.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	RBCK1
Full Name	Ranbp2-Type And C3Hc4-Type Zinc Finger Containing 1
Alternative Name	XAP3; XAP4; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; HOIL-1; ZRANB4; C20orf18; UBCE7IP3
Location	20p13
Gene ID	10616
Summary	The encoded protein has functions including protein sequestration, transcriptional coactivation, and ubiquitin binding. It participates in NF-kappaB signaling and protein ubiquitination and is part of the LUBAC complex. It has been implicated in glycogen storage disorders.