GlycoFlux™ Human TPI1 Overexpression HEK293T Cell Line (CAT#: GLJF-0925-JF602)

Description	GlycoFlux™ human TPI1 overexpression HEK293T cell line is a stable cell line engineered to produce TPI1, a glycolytic enzyme catalyzing the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Mutations lead to triosephosphate isomerase deficiency, a rare metabolic disorder with anemia and neurological manifestations. With pseudogenes on multiple chromosomes and alternative splicing variants, this model supports research in glycolysis regulation, red blood cell metabolism, and neurometabolic disease.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	TPI1
Full Name	Triosephosphate Isomerase 1
Alternative Name	TIM; TPI; TPID; HEL-S-49
Location	12p13.31
Gene ID	7167
Summary	The encoded enzyme catalyzes the reversible interconversion between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate in glycolysis and gluconeogenesis. Mutations cause triosephosphate isomerase deficiency. Pseudogenes are found on chromosomes 1, 4, 6, and 7. Alternative splicing generates multiple forms.