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Quantitative Human GRIA3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX411

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Specifications
Product Description
The quantitative human GRIA3 (glycosylated) sandwich ELISA kit is designed to detect human glutamate receptor, ionotropic, AMPA3 (GRIA3) levels. GRIA3 is a subunit of the AMPA receptor, which contributes to fast synaptic transmission in the central nervous system. While its specific role may differ slightly from other subunits, GRIA3 is essential for proper neuronal signaling. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of GRIA3 in the sample.
Target
GRIA3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glutamate receptor, ionotropic, AMPA3
Alternate Names
GRIA3; Glutamate receptor, ionotropic, AMPA3; GLUR3; GLURC; AMPA-selective glutamate receptor 3
Uniprot No.
N-Glycosylation Site
63, 266, 380, 415, 422
Application Notes
Application
The quantitative human GRIA3 (glycosylated) sandwich ELISA kit is used to measure glycosylated GRIA3 levels in samples. This measurement is valuable in neuroscience research, particularly in studies of synaptic functions and neurological diseases. The kit is applied in investigations of conditions where AMPA receptor dysregulation is implicated.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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