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Quantitative Mouse GRIA3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX916

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Specifications
Product Description
The quantitative mouse GRIA3 (glycosylated) sandwich ELISA kit is designed to detect mouse glutamate receptor, ionotropic, AMPA3 (GRIA3) levels. GRIA3 is a subunit of the AMPA receptor. AMPA receptors are ligand-gated ion channels in the central nervous system. They mediate fast synaptic transmission, which is crucial for brain functions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.083 ng/mL, which can accurately detect low concentrations of GRIA3 in the sample.
Target
GRIA3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.083 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Mouse
Full Name
Glutamate receptor, ionotropic, AMPA3
Alternate Names
GRIA3; Glutamate receptor, ionotropic, AMPA3; GLUR3; GLURC; AMPA-selective glutamate receptor 3
Uniprot No.
N-Glycosylation Site
57, 260, 374, 409, 416
Application Notes
Application
The quantitative mouse GRIA3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated GRIA3 levels in biological samples. The assay is valuable for investigating the role of GRIA3 in synaptic transmission, learning, and neurological disorders.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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