Creative Biolabs-Immuno-oncology

T Cell based In Vitro Assay Service

Creative Biolabs has developed a variety of T and B cell-based assays to analyze cancer antigens and identify immunodominant epitopes based on extensive, versatile techniques. The in vitro assay is a well-established and reproducible method for characterizing epitope-induced T cell responses, such as cytotoxicity, cell proliferation, and suppression function. Based on your sample, our experienced scientists can provide proper assays to accelerate your target-mediated immunotherapy development.

Introduction What We Can Offer Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us

Introduction of T Cell-based In Vitro Assay

T cell-based in vitro assays are powerful tools for evaluating the function of T cells, key players in the adaptive immune system. They are widely used to assess the efficacy and mechanism of action of immunotherapies, vaccines, and treatments for autoimmune diseases. These assays provide a controlled environment to study T cell activation, proliferation, and differentiation. Their importance is underscored by their frequent use in research publications and their role in validating therapeutic targets before in vivo studies.

Explore the specific applications of our solutions to your research objectives. - Request a consultation.

What We Can Offer

Cytokine Release Assays

Techniques Functions Advantages Limitations
ELISA Detect Cytokine Secretion
  • Sensitive
  • Accurate
  • Widely Used
  • Laborious
  • Limited Detection Range
Advanced ELISA Detect Cytokine Secretion
  • High Throughput
  • Greater Sensitivity
  • Wide Range of Detection Limits
  • Free of Washing Steps
None

Cell Proliferation Assays

Technology Principle Correlate Techniques Advantages Limitations
CFSE Assay Reduced CFSE Signal as Cell Division Flow Cytometry
  • Simple to Perform
  • Relatively High Throughput
  • Non-radioactivity
May Influence Cell Viability
3H-thymidine Assay Incorporate in Dividing Cells Liquid Scintillation Counter
  • High Throughput
  • Sensitive
Radioactive
BrdU Assay Incorporate in Dividing Cells Flow Cytometry
Colorimetric Method
  • Specific and Direct
  • High Specificity
Radioactive
51Cr Assay Chromium-51 Release Gamma Counter
Liquid Scintillation Counter
  • Sensitive
  • Reflect Cytotoxicity
Radioactivity

T Cell Suppression Assays

Regulatory T cells (Treg) are important immune cells that can control immune activation, maintain the balance of the host immune system, and prevent self-immune responses. It has been demonstrated that antigen-induced Treg cells with higher CD25 expression are derived from the same clone as corresponding CD25low effector T cells (Teff) and possess the same epitope-specific TCR sequences.

Highlights

Scientific Excellence

Our team provides deep scientific expertise and high-quality data to help you interpret results and make informed decisions. We focus on rigorous methodology for maximum reliability and scientific integrity.

Client-Centric Approach

We partner with you to understand your specific project needs. Our solutions are tailored to your requirements, ensuring a collaborative and supportive experience from initial consultation to the final report.

Service Features

Predictive Power

By leveraging advanced technologies and physiologically relevant models, our assays generate data with high predictive value for your research. This helps you identify lead candidates with confidence.

Decisive Edge

Our services are designed to give you a decisive advantage in drug development. We help accelerate your timeline and advance your therapeutic candidates with actionable data and expert analysis.

Discover how a partnership with us can streamline your research - Get a quote today.

Customer Reviews

FAQs

How do your assays compare to other methods?

Our assays leverage advanced technologies like live-cell imaging and multiplex analytical platforms, offering superior sensitivity and a more comprehensive view of the immune response than traditional methods. We prioritize reproducibility and provide detailed data, helping you to make more informed decisions.

How do you handle sample variability?

We use standardized protocols and rigorous quality control measures for all assays. We can also provide services to test your compound across a panel of different donors, which helps to account for biological variability and strengthen your data.

Related Services

T Cell-based Cytometric Bead Array Assay

This sensitive method detects multiple cytokines simultaneously, offering a quantitative and comprehensive profile of the immune response for your research project.

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T and B Cell-based ELISA Assay

A versatile and quantitative assay for a variety of analytes. It provides a reliable method to measure protein or antibody levels in your samples.

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T and B Cell-based ELISPOT Assay

Our service detects and enumerates cytokine-secreting cells at the single-cell level. This offers a powerful and high-resolution view of the immune response.

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T Cell-based High Throughput Multiplexed Assay

An efficient and scalable solution for screening a large number of samples against a wide range of targets. This accelerates your discovery timeline.

Learn More →

Cell Line Development

For projects involving the development of therapeutic antibodies, our protein engineering services offer the expertise needed to create highly specific and effective candidates.

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How to Contact Us

Creative Biolabs provides the scientific clarity and technical expertise you need to accelerate your research and development. Our comprehensive T cell-based in vitro assay services are designed to deliver precise, reliable, and actionable data. We are committed to being your strategic partner in advancing the next generation of therapeutics.

Contact us for more information and to discuss your project.

For Research Use Only | Not For Clinical Use

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