GlycoErase™ ENO3 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF390)

Description	GlycoErase™ ENO3 knockout HEK293 cell line is a human embryonic kidney (HEK293) model lacking ENO3 expression. ENO3 encodes a muscle-specific enolase isoenzyme that plays roles in skeletal muscle energy metabolism and regeneration. Mutations in ENO3 are linked to glycogen storage diseases and muscle dysfunction. The knockout of ENO3 in the HEK293 background offers a system for studying glycolysis, energy regulation, and muscle-associated pathways in a human cellular context, supporting applications in neuromuscular disease research, metabolic disorders, and therapeutic development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ENO3
Full Name	Enolase 3
Alternative Name	MSE; GSD13
Location	17p13.2
Gene ID	2027
Summary	Beta-enolase is found in adult skeletal muscle and may contribute to muscle development and regeneration. A developmental switch from alpha-enolase to beta-enolase occurs in rodent muscle. Mutations cause glycogen storage disease. Multiple isoforms arise via alternative splicing.