GlycoErase™ G6PC1 Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF509)

Description	GlycoErase™ G6PC1 knockout HEK293T cell line is a human embryonic kidney-derived model in which G6PC1 expression has been eliminated. G6PC1 encodes the catalytic subunit of glucose-6-phosphatase, catalyzing the final step of gluconeogenesis and glycogenolysis. Its loss in HEK293T cells provides a system to study hepatic-like glucose production pathways, altered acetyl-CoA flux, and metabolic dysregulation relevant to glycogen storage disease type I, supporting investigations in metabolic control and therapeutic strategy development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	G6PC1
Full Name	Glucose-6-Phosphatase Catalytic Subunit 1
Alternative Name	G6PC; G6PT; GSD1; GSD1a; G6Pase
Location	17q21.31
Gene ID	2538
Summary	Glucose-6-phosphatase (G6PC) catalyzes the conversion of glucose-6-phosphate to glucose and phosphate, which is critical in glucose homeostasis, gluconeogenesis, and glycogenolysis. Mutations cause glycogen storage disease type I (von Gierke disease).