GlycoErase™ PGM3 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF433)

Description	GlycoErase™ PGM3 knockout HEK293 cell line is a human embryonic kidney (HEK293) model lacking PGM3 expression. PGM3 encodes a phosphohexose mutase that interconverts glucose-1-phosphate and glucose-6-phosphate, linking glycogen metabolism with nucleotide sugar biosynthesis. Variants influence susceptibility to diabetic complications and immune dysregulation. This knockout provides a system for investigating carbohydrate flux, immune-metabolic pathways, and diabetic nephropathy mechanisms in a human cellular background.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PGM3
Full Name	Phosphoglucomutase 3
Alternative Name	AGM1; PAGM; IMD23; PGM 3
Location	6q14.1
Gene ID	5238
Summary	This gene encodes a member of the phosphohexose mutase family, mediating both glycogen formation and utilization by interconverting glucose-1-phosphate and glucose-6-phosphate. A SNP in this gene may affect diabetic nephropathy resistance. Multiple isoforms exist due to alternative splicing.