GlycoErase™ PHKG1 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF670)

Description	GlycoErase™ PHKG1 knockout HepG2 cell line is a model lacking PHKG1 expression. PHKG1 encodes a gamma subunit isoform of phosphorylase kinase, crucial for glycogenolytic enzyme activity. This knockout enables studies on glycogen metabolism, kinase activity, and calcium-dependent regulation in the liver.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKG1
Full Name	Phosphorylase Kinase Catalytic Subunit Gamma 1
Alternative Name	PHKG
Location	7p11.2
Gene ID	5260
Summary	The encoded protein is a serine/threonine kinase with a single kinase domain and two calmodulin-binding regions. It serves as the catalytic element of the phosphorylase kinase holoenzyme, a 16-part complex critical for glycogen breakdown. Pseudogenes appear on 7q11.21 and 11p11.12, and multiple mRNA variants exist.