GlycoErase™ PPP1CA Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF441)

Description	GlycoErase™ PPP1CA knockout HEK293 cell line is a HEK293 model without PPP1CA expression. PPP1CA encodes the alpha catalytic subunit of protein phosphatase 1 (PP1), a serine/threonine phosphatase that regulates cell cycle, glycogen metabolism, and cardiac function. Dysregulation contributes to diabetes, cancer, and heart failure. This knockout provides a versatile platform for oncology, cardiovascular signaling, and therapeutic phosphatase regulation in a human context.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1CA
Full Name	Protein Phosphatase 1 Catalytic Subunit Alpha
Alternative Name	PP1A; PP-1A; PPP1A; PP1alpha
Location	11q13.2
Gene ID	5499
Summary	This gene encodes one of three catalytic subunits of protein phosphatase 1 (PP1), the alpha subunit, which associates with over 200 regulatory proteins to form specific holoenzymes. PP1 regulates processes including cell division, glycogen metabolism, muscle contraction, protein synthesis, and viral transcription. Dysregulation is implicated in heart failure, diabetes, and cancer.