GlycoErase™ PPP1CA Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF673)

Description	GlycoErase™ PPP1CA knockout HepG2 cell line eliminates PPP1CA, encoding the alpha catalytic subunit of protein phosphatase 1 (PP1). PP1 regulates cell division, glycogen metabolism, protein synthesis, and muscle contractility. PP1 deregulation is linked to diabetes, cancer, and heart disease. This knockout model offers a system for exploring oncology, metabolism, and cardiovascular biology.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1CA
Full Name	Protein Phosphatase 1 Catalytic Subunit Alpha
Alternative Name	PP1A; PP-1A; PPP1A; PP1alpha
Location	11q13.2
Gene ID	5499
Summary	This gene produces the alpha catalytic subunit of protein phosphatase 1 (PP1), which works with numerous regulatory proteins to direct dephosphorylation of specific targets. PP1 participates in controlling glycogen metabolism, muscle contraction, mitosis, protein synthesis, and more. Its dysregulation has been linked to heart failure, diabetes, and cancer.