Description

GlycoFlux™ Lenti-A4GALT Viral Particle is a ready-to-use, replication-defective lentiviral preparation encoding A4GALT (α1,4-galactosyltransferase), which transfers galactose to lactosylceramide to generate globotriaosylceramide (Gb3/CD77) associated with the P(k) blood-group antigen. The vector enables stable A4GALT overexpression across mammalian systems-including human adherent cancer lines, hematologic/immune suspension cells, common animal lines, primary cells, stem cells, and 3D models/organoids-supporting robust induction of Gb3 biosynthesis and surface display for glycosphingolipid pathway studies and functional assays. Supplied ready for transduction, effective in both dividing and non-dividing cells, and compatible with antibiotic selection or FACS enrichment.

Product Type

Recombinant Lentivirus

Target

A4GALT

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Full Name	Alpha 1,4-galactosyltransferase (P1PK blood group)
Alternative Name	A14GALT; P(k); P1; Gb3S
Location	22q13.2
Gene ID	53947
Summary	Transfers galactose to lactosylceramide, producing globotriaosylceramide associated with P(k) blood group antigen.

GlycoFlux™ Lenti-Human A4GALT Viral Particle (CAT#: GLJF-1025-JF1)

Specifications

Quality Control

Target Information

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