GlycoFlux™ AAV1-Human A4GALT Viral Particle

GlycoFlux™ AAV1-A4GALT Viral Particle delivers robust A4GALT expression via adeno-associated virus serotype 1 (AAV1), catalyzing galactose transfer to lactosylceramide to generate globotriaosylceramide (Gb3), the P(k) antigen of the P blood group system. Engineered on AAV1, this vector shows strong tissue tropism for smooth muscle, the central nervous system (CNS), retina, pancreas, inner ear, heart, and liver-enabling efficient in vitro and in vivo transduction across these targets. Ideal for glycosphingolipid pathway engineering and controlled display of Gb3 epitopes, it supports long-term expression in post-mitotic tissues and is supplied as a replication-incompetent, research-use-only preparation.

Recombinant AAV1

A4GALT

Human

CMV, CAG, TRE, or other promoters

Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver

AAV Triple-Plasmid Transfection System

HEK293T

This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.

Shipped on dry ice, with temperature maintained at ≤ -80°C.

Store at -80°C to preserve viral titer and stability.

Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).

This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.

qPCR/TCID50

No mycoplasma contamination was detected.

Sterility testing verified the absence of microbial contamination.

Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.

All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.

Alpha 1,4-galactosyltransferase (P1PK blood group)

A14GALT; P(k); P1; Gb3S

22q13.2

Catalyzes the transfer of galactose to lactosylceramide, forming globotriaosylceramide, which is associated with the P(k) antigen of the P blood group system.