Description

GlycoFlux™ MSCV-A4GALT Viral Particle employs a Murine Stem Cell Virus (MSCV) γ-retroviral backbone to drive stable, high-level A4GALT expression in dividing mammalian cells-particularly suited to hematopoietic and stem/progenitor systems. Increased A4GALT activity transfers galactose to lactosylceramide to form globotriaosylceramide (Gb3), the P(k) blood group antigen, enabling programmable Gb3 surface display for glycosphingolipid pathway engineering, P(k) receptor-ligand studies, and variant or dose-response benchmarking in durable cell lines.

Product Type

Recombinant MSCV Retrovirus

Target

A4GALT

Species

Human

Promotor

MSCV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.

Shipping

Dispatched on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to preserve titer and stability.

Handling Notes

Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation	All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.

Full Name	Alpha 1,4-galactosyltransferase (P1PK blood group)
Alternative Name	A14GALT; P(k); P1; Gb3S
Location	22q13.2
Gene ID	53947
Summary	Transfers galactose to lactosylceramide, forming the globotriaosylceramide antigen of the P(k) blood group.

GlycoFlux™ MSCV-Human A4GALT Viral Particle (CAT#: GLJF-1025-JF515)

Specifications

Quality Control

Target Information

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