GlycoErase™ GBE1 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF630)

Description	GlycoErase™ GBE1 knockout HepG2 cell line removes GBE1, encoding the glycogen branching enzyme that introduces α-1,6 linkages to ensure glycogen solubility and energy balance. GBE1 mutations cause glycogen storage disease IV (Andersen's disease). This knockout supports studies of glycogen structure, liver storage diseases, and metabolic homeostasis, providing a system for therapeutic validation in glycogen disorders.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GBE1
Full Name	1,4-Alpha-Glucan Branching Enzyme 1
Alternative Name	GBE; APBD; GSD4
Location	3p12.2
Gene ID	2632
Summary	This gene encodes glycogen branching enzyme, which transfers alpha-1,4-linked glucosyl units to create alpha-1,6 branches in glycogen, increasing solubility. Mutations cause glycogen storage disease IV (Andersen's disease).