GlycoFlux™ Human GBE1 Overexpression Jurkat Cell Line

GlycoFlux™ human GBE1 overexpression Jurkat cell line is a stable cell line engineered to express glycogen branching enzyme 1 (GBE1), which catalyzes the formation of alpha-1,6 branches in glycogen, improving solubility and reducing osmotic stress. Mutations in GBE1 cause glycogen storage disease IV (Andersen disease). This model provides a tool for dissecting glycogen structure-function relationships and for investigating branching enzyme deficiencies in metabolic and immunological contexts.

Overexpression Cell Lines

Human

Suspension-growing

1:3~1:5

Jurkat cell line

T-cell acute lymphoblastic leukemia (T-ALL)

T Lymphocyte

T-cell acute lymphoblastic leukemia (T-ALL)

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & RPMI

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

GBE1

1,4-Alpha-Glucan Branching Enzyme 1

GBE; APBD; GSD4

3p12.2

The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).