L-Arginine-HCl, ¹³C₆, ¹⁵N₄ for SILAC (CAT#: GLJF-0225-HX29)

Product Description	L-Arginine-HCl, 13C6, 15N4 for SILAC is a stable isotope-labeled form of the essential amino acid L-arginine. In this compound, all six carbon atoms are replaced with carbon-13 isotopes, and all four nitrogen atoms are replaced with nitrogen-15 isotopes. Incorporating this labeled arginine into cell culture media allows cells to incorporate the heavier isotopes into all newly synthesized proteins. This creates a distinct mass difference compared to proteins from cells grown in standard media, enabling researchers to distinguish and quantify relative protein abundance between different experimental conditions using mass spectrometry.
CAS	202468-25-5
Synonyms	202468-25-5; MFCD04118221; (2S)-2-(15N)Azanyl-5-[bis(15N)(azanyl)(113C)methylideneamino](1,2,3,4,5-13C5)pentanoic acid;hydrochloride; L-Arginine-13C6,15N4 hydrochloride
Formula	C6H15ClN4O2
MDL No.	MFCD04118221
MW	220.59
InChI	InChI=1S/C6H14N4O2.ClH/c7-4(5(11)12)2-1-3-10-6(8)9;/h4H,1-3,7H2,(H,11,12)(H4,8,9,10);1H/t4-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1,8+1,9+1,10+1;
InChI Key	KWTQSFXGGICVPE-YAUSPDTOSA-N
Purity	Min. 95%
Identity	Conforms to structure
Storage	Store at 2-8°C for long term storage.

Application

L-Arginine-HCl, 13C6, 15N4 is specifically designed for use in stable isotope labeling by amino acids in cell culture (SILAC) experiments. SILAC is a powerful quantitative proteomics technique that incorporates non-radioactive, stable isotope-labeled amino acids into the proteins of living cells. By culturing cells in media containing this labeled L-arginine, researchers can achieve uniform labeling of newly synthesized proteins. Mass spectrometry can then distinguish between labeled and unlabeled peptides based on their mass differences, allowing for precise quantification of protein expression levels between different experimental conditions. This approach is invaluable for studying protein dynamics and identifying biomarkers.