L-Proline for SILAC

L-Proline for SILAC is the standard, unlabeled form of the amino acid L-proline. This unlabeled L-proline is incorporated into the proteins of cells grown in standard media, establishing the "light" proteome. This light proteome acts as the reference against which the "heavy" (labeled) proteome from cells grown in media containing stable isotope-labeled proline is compared using mass spectrometry. This comparison enables researchers to quantify relative changes in protein abundance between different experimental conditions.

147-85-3

147-85-3; MFCD00064318; (2S)-Pyrrolidine-2-carboxylic acid; H-Pro-OH; EC 205-702-2; (S)-2-Pyrrolidinecarboxylic acid; L-proline

C₅H₉NO₂

MFCD00064318

115.13

InChI=1S/C5H9NO2/c7-5(8)4-2-1-3-6-4/h4,6H,1-3H2,(H,7,8)/t4-/m0/s1

ONIBWKKTOPOVIA-BYPYZUCNSA-N

Min. 95%

Conforms to structure

Store at 2-8°C for long term storage.

L-Proline for SILAC plays a crucial role in quantitative proteomics experiments utilizing stable isotope labeling by amino acids in cell culture (SILAC). As the standard, unlabeled form of proline, it serves as the essential component for establishing the "light" proteome in SILAC experiments. L-Proline for SILAC is fundamental for investigating alterations in protein expression, post-translational modifications, and protein-protein interactions, especially when focusing on processes involving proline or proline-rich regions of proteins.