L-Leucine for SILAC (CAT#: GLJF-0225-HX30)

Product Description	L-Leucine for SILAC is the standard, unlabeled form of the essential amino acid L-leucine, used as a control or "light" component in stable isotope labeling by amino acids in cell culture (SILAC) experiments. While SILAC relies on comparing cells grown in media containing isotopically labeled amino acids, a baseline is essential. This unlabeled L-leucine is incorporated into the proteins of cells grown in standard media, establishing the "light" proteome. This light proteome acts as the reference against which the "heavy" (labeled) proteome from cells grown in media containing stable isotope-labeled leucine is compared using mass spectrometry. This comparison enables researchers to quantify relative changes in protein abundance between different experimental conditions.
CAS	61-90-5
Synonyms	61-90-5; MFCD00002617; (2S)-2-Amino-4-methylpentanoic acid; L-(+)-Leucine; Pentanoic acid, 2-amino-4-methyl-, (S)-; EC 200-522-0
Formula	C6H13NO2
MDL No.	MFCD00002617
MW	131.17
InChI	InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1
InChI Key	ROHFNLRQFUQHCH-YFKPBYRVSA-N
Purity	Min. 95%
Identity	Conforms to structure
Storage	Store at 2-8°C for long term storage.

Application

L-Leucine for SILAC is crucial for quantitative proteomics studies, enabling accurate comparison of protein expression levels between different cell states or treatment conditions. This technique provides scientists with valuable insights into cellular processes, disease mechanisms, and potential therapeutic targets.