L-Leucine for SILAC

L-Leucine for SILAC is the standard, unlabeled form of the essential amino acid L-leucine, used as a control or "light" component in stable isotope labeling by amino acids in cell culture (SILAC) experiments. While SILAC relies on comparing cells grown in media containing isotopically labeled amino acids, a baseline is essential. This unlabeled L-leucine is incorporated into the proteins of cells grown in standard media, establishing the "light" proteome. This light proteome acts as the reference against which the "heavy" (labeled) proteome from cells grown in media containing stable isotope-labeled leucine is compared using mass spectrometry. This comparison enables researchers to quantify relative changes in protein abundance between different experimental conditions.

61-90-5

61-90-5; MFCD00002617; (2S)-2-Amino-4-methylpentanoic acid; L-(+)-Leucine; Pentanoic acid, 2-amino-4-methyl-, (S)-; EC 200-522-0

C₆H₁₃NO₂

MFCD00002617

131.17

InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1

ROHFNLRQFUQHCH-YFKPBYRVSA-N

Min. 95%

Conforms to structure

Store at 2-8°C for long term storage.

L-Leucine for SILAC is crucial for quantitative proteomics studies, enabling accurate comparison of protein expression levels between different cell states or treatment conditions. This technique provides scientists with valuable insights into cellular processes, disease mechanisms, and potential therapeutic targets.