CRM197 Protein (E. coli) (VCar-Lsx005)
| CAT | Size | Price | Quantity | |
|---|---|---|---|---|
| VCar-Lsx005 |
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CRM197, which is widely used as a carrier protein in conjugate vaccines, was expressed in E. coli, and purified by state-of-art protein purification method such as centrifugation, amonium sulfate precipitation and chromatography, then lyophilized after addition of sucrose.
Datasheet
Specifications
Source
E. coli
Target
Glu52 Diptheria Toxin Antibody
Type
Proteins
Applications
Used for detection of antibodies or as a carrier protein for conjugate vaccines.
Quantity
Before lyophilization, CRM197(aq) was about 5mg/ml in sterile water with 5% sucrose; Vials containing appoximately 5mL of CRM197(aq) were lyophilized.
Purity
> 90% pure by SDS-PAGE.
Antigen Information
Introduction
CRM197 is a non-toxic mutant of diphtheria toxin, currently used as a carrier protein for polysaccharides and haptens to make them immunogenic. CRM197 is a genetically detoxified form of diphtheria toxin. A single mutation at position 52, substituting glutamic acid for glycine, causes the ADP-ribosyltransferase activity of the native toxin to be lost. CRM197 is widely used as a carrier protein for conjugate vaccines, because it is genetically detoxified and retains its full complement of lysine amines for conjugation. There is also evidence suggesting that, compared with tetanus toxoid, there is less carrier-induced suppression of the immune response, especially when there are many individual polysaccharides linked to the same carrier protein.
Alternative Names
CRM197; Glu52 diptheria toxin; Cross-reactive materials 197
Related Product
All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.
