0
Inquiry Basket

There is no product in the shopping cart, buy it!

L-Lysine-2HCl, 13C6, 15N2 for SILAC (CAT#: GLJF-0225-HX25)

Datasheet
SizeQtyAdd To Basket
50 mg
100 mg
250 mg
500 mg

Product Description L-Lysine-2HCl, 13C6, 15N2 for SILAC is a stable isotope-labeled form of the essential amino acid L-lysine, where all six carbon atoms are replaced with the stable isotope carbon-13, and both nitrogen atoms are replaced with nitrogen-15. Supplied as its dihydrochloride salt for enhanced solubility and stability, this compound is specifically designed for use in stable isotope labeling by amino acids in cell culture (SILAC) experiments. Incorporating this heavier, doubly labeled lysine into cell culture media results in the metabolic incorporation of the stable isotopes into all newly synthesized proteins. This introduces a distinct mass shift compared to proteins from cells grown in standard media, enabling researchers to differentiate and quantify relative protein abundance between different cell populations or experimental conditions using high-resolution mass spectrometry. The dual labeling with both 13C and 15N further enhances the mass difference, improving the accuracy and clarity of protein quantification in SILAC experiments, and is particularly useful in complex experimental designs.
CAS 202406-54-0
Synonyms 202406-54-0; L-Lysine-2HCl, 13C6, 15N2; L-Lysine-13C6,15N2 (dihydrochloride); (2S)-2,6-Bis(15N)(azanyl)(1,2,3,4,5,6-13C6)hexanoic acid
Formula C6H16C12N2O2
MW 227.05
InChI InChI=1S/C6H14N2O2.2ClH/c7-4-2-1-3-5(8)6(9)10;;/h5H,1-4,7-8H2,(H,9,10);2*1H/t5-;;/m0../s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1,8+1;
InChI Key JBBURJFZIMRPCZ-XLWLNXBUSA-N
Purity Min. 95%
Identity Conforms to structure
Storage Store at 2-8°C for long term storage.
Application L-Lysine-2HCl, 13C6, 15N2 for SILAC is primarily used in quantitative proteomics experiments employing stable isotope labeling by amino acids in cell culture (SILAC). This doubly labeled lysine allows researchers to metabolically incorporate a distinct mass tag into all proteins synthesized by cells grown in media containing it. It is a crucial tool for advancing our understanding of cellular processes, disease mechanisms, and the development of novel therapeutic strategies.
For Research Use Only.
Online Inquiry
  • (USA)
    (UK)
    (Germany)
  • Contact Us
  • Global Locations
Follow us on
  • (USA)
    (UK)
    (Germany)
ISO 9001 Certified - Creative Biolabs Quality Management System.