L-Lysine-2HCl for SILAC

L-Lysine-2HCl for SILAC is a form of the essential amino acid L-lysine specifically prepared for use in stable isotope labeling by amino acids in cell culture (SILAC). While it's the standard, unlabeled form of lysine, it serves as the counterpart to its stable isotope-labeled versions (like ¹³C₆-lysine). In SILAC experiments, researchers often use both labeled and unlabeled lysine in separate cell populations to establish a baseline for comparison. The unlabeled L-lysine-2HCl helps define the "light" proteome, against which the "heavy" (labeled) proteome is compared in mass spectrometry analysis. This comparison allows for precise quantification of relative protein abundance changes between different experimental conditions.

657-26-1

657-26-1; MFCD00066389; L-Lysine-2HCl; L-Lysine dihydrochloride; Lysine dihydrochloride; (S)-2,6-Diaminohexanoic acid dihydrochloride; L-(+)-Lysine dihydrochloride

C₆H₁₆C₁₂N₂O₂

MFCD00066389

219.11

InChI=1S/C6H14N2O2.2ClH/c7-4-2-1-3-5(8)6(9)10;;/h5H,1-4,7-8H2,(H,9,10);2*1H/t5-;;/m0../s1

JBBURJFZIMRPCZ-XRIGFGBMSA-N

Min. 95%

Conforms to structure

Store at 2-8°C for long term storage.

The use of L-Lysine-2HCl for SILAC facilitates accurate, reproducible, and high-throughput analysis of complex protein mixtures, advancing research in fields such as cell biology, molecular biology, and biomedical sciences.