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Size | Qty | Add To Basket |
---|---|---|
1 μg | ||
2 μg |
Product Description | Asp-N protease (sequencing grade) is a sequencing grade endoprotease that specifically cleaves peptide bonds N-terminal to aspartic acid (Asp) and glutamic acid (Glu) residues. The enzyme exhibits optimal activity in the pH range of 4.0 to 9.0, making it suitable for a wide range of experimental conditions. The enzyme can be used alone or in combination with other proteases to improve protein digestion efficiency. Asp-N is suitable for both in-solution and in-gel digestion processes. |
Synonyms | Asparagine-specific endopeptidase, sequencing grade; Asp-N endopeptidase, sequencing grade; Sequencing grade Asp-N protease; Asp-N protease for protein sequencing; Protein sequencing grade Asp-N |
MW | 24.5 kDa |
Source | Pseudomonas fragi |
Purity | Min. 98% |
Form | Lyophilized powder |
Storage | Store at -20°C for long term storage. |
Application | Asp-N protease (sequencing grade) is widely used in proteomics and protein sequencing. It is used for enzymatic treatment of protein samples before mass spectrometry analysis to generate specific peptides, which helps to improve the accuracy and resolution of mass spectrometry analysis. By using Asp-N, researchers can more effectively perform protein structural analysis, post-translational modification identification, and protein interaction research, thereby promoting the development of biological and medical research. |