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| Size | Qty | Add To Basket |
|---|---|---|
| 500 mL | ||
| 1 L |
| Product Description | Powdered RPMI 1640 media for SILAC is a specialized cell culture medium designed for use in stable isotope labeling by amino acids in cell culture (SILAC) experiments. This powdered formulation of RPMI 1640 medium is devoid of the amino acids lysine and arginine, which are essential for protein synthesis. By omitting these amino acids, researchers can supplement the medium with isotopically labeled versions, such as 13C- or 15N-labeled lysine and arginine. This allows cells grown in the medium to incorporate the labeled amino acids into newly synthesized proteins. The resulting isotopic labeling facilitates precise quantitative proteomic analyses using mass spectrometry. By providing RPMI 1640 in a powdered format, storage and handling are simplified, while enabling researchers to tailor the medium to their specific needs, thereby enhancing the accuracy and reliability of quantitative proteomic analyses in various biological research applications. |
| Synonyms | Powdered RPMI 1640 media for SILAC; RPMI 1640 powder for SILAC; RPMI 1640 in powder form for stable isotope labeling; RPMI 1640 powdered cell culture medium for SILAC |
| Form | Powder |
| Storage | Store at 2-8°C for long term storage. |
| Application | Powdered RPMI 1640 media for SILAC offers researchers a flexible and customizable solution for conducting stable isotope labeling by amino acids in cell culture (SILAC) experiments. The powdered medium ensures that cells are grown under controlled conditions without interfering unlabeled amino acids, leading to more accurate mass spectrometry results. This product is invaluable for studies in protein expression, post-translational modifications, and interaction dynamics within various fields of biomedical research. |