High-Throughput Glycomic Analysis Services

What Is High-Throughput Glycomic Analysis?

High-throughput glycomic analysis refers to the rapid, parallelized profiling of glycan structures across hundreds or thousands of biological samples. By integrating automated workflows, sensitive analytical platforms, and advanced bioinformatics, this approach enables researchers to systematically investigate glycosylation dynamics at scale. At Creative Biolabs, we have established a fully integrated high-throughput glycomic analysis platform, combining:

• Ultra-High-Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD)
• Fluorescence-Labeled Lectin-Based Glycan Assay
• Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)
• Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry (LC–ESI–MS/MS)

These technologies allow us to identify, quantify, and compare glycan structures with precision across hundreds of samples per day. From therapeutic antibody characterization to biomarker discovery and clone screening, our high-throughput glycomics service enables researchers to investigate glycosylation at unprecedented scale, depth, and resolution.

Glycomic Analysis Workflow

1. Sample submission and quality assessment
2. Enzymatic or chemical glycan release
3. Fluorescent labeling or derivatization
4. Glycan separation by UHPLC-FLD or other appropriate platforms
5. Mass spectrometry or lectin-based glycan assay
6. Automated glycopeak identification using glycosylation databases
7. Quantification, clustering, and interpretation
8. Delivery of comprehensive reports

Fig.2 Workflows for high-throughput glycomic analysis.¹

Integrated Analytical Technologies for Glycomic Analysis

Our platform is fully compatible with sialic acid-specific derivatization techniques, glycan permethylation, and HILIC-based enrichment protocols to enhance detection of labile structures and low-abundance glycoforms.

Sample preparation optimization

Our pipeline prioritizes high recovery, uniform processing, and compatibility with multiple sample types (serum, cell culture supernatants, purified glycoproteins):

• Parallel glycoprotein digestion using optimized enzyme batches (e.g., PNGase F, β-elimination)
• Removal of interfering RNA/DNA using chaotropic salts and organic solvents in 96-well format
• Derivatization and purification protocols validated across >10,000 samples for robustness
• Internal glycan standards included to support inter-plate and inter-batch normalization

Deliverables

Each project includes:

• Annotated glycan peak lists (monosaccharide composition, molecular weight, retention time)
• Quantitative plots comparing relative abundance across groups
• Glycosylation pattern clustering
• Site-specific glycopeptide data if MS-based profiling is used
• Technical and biological replicates with CV statistics
• Raw data files and visualized chromatograms or spectra
• Recommendations for next-step glycoengineering or QC validation

High-throughput Titer Assays for Accelerated Clone Selection

To complement our high-throughput glycan screening capabilities, Creative Biolabs has developed robust, automation-friendly titer assay platforms tailored for antibody and Fc-fusion protein quantification in early-stage development.

Antibody/Fc Fusion Protein Titer Assays

Our optimized titer assay supports the direct quantification of antibodies and Fc-fusion proteins in microvolumes of culture supernatant. The assay runs in a 384-well plate format and is fully automated, delivering results within 25 minutes—without requiring any sample pretreatment. This makes it ideal for high-throughput clone evaluation with minimal hands-on time.

High-Throughput Titer Assays for Cell Line Development

Designed specifically for large-scale screening campaigns, our automated titer assay system combines high speed, excellent reproducibility, and cost efficiency. It enables rapid ranking of hundreds of candidate clones based on expression levels, supporting cell line development and upstream process optimization.

By integrating titer determination and glycan profiling within the same high-throughput system, Creative Biolabs empowers researchers to:

• Correlate productivity with glycosylation quality at early stages
• Identify high-producing clones with desirable glycan traits (e.g., afucosylation, sialylation)
• Shorten development timelines while improving candidate selection confidence

With extensive expertise in glycomics and titer analytics, Creative Biolabs offers a powerful platform to streamline biologics development—from screening to scale-up. For more details, please contact our team or submit a direct inquiry.

Talk to an Expert

Applications

Biopharmaceutical QC and Clone Selection

• Detects glycosylation changes in mAbs, Fc-fusion proteins, and biosimilars across production batches.
• Supports clone selection by profiling sialylation, core fucosylation, and galactosylation levels.

Biomarker Discovery and Clinical Glycomics

• Identifies disease-specific glycan features such as increased tri-antennary structures in cancer or reduced sialylation in autoimmune diseases.
• Allows cross-cohort statistical comparison for high-sample clinical studies.

Glycoengineering Strategy Evaluation

• Validates metabolic and genetic interventions aimed at remodeling glycosylation pathways.
• Supports characterization of glycosylation changes during host cell or enzyme system modification.

Why choose Creative Biolabs?

• High-throughput scalability: As short as 1 week for up to 100 samples for glycan screening using automated UHPLC-FLD.
• Multi-platform integration: Choose from UHPLC-FLD, LC-MS/MS, or lectin-based screening based on resolution needs.
• Broad compatibility: Accepts samples from mammalian, microbial, plant, or synthetic sources.
• Comprehensive structural depth: Our in-house bioinformatics tools match >150,000 glycan structures across major glycosylation databases.

For a customized project proposal or consultation with our glycomics experts, please contact us to accelerate your glycoprotein projects with leading analytical precision and custom-tailored solutions.

FAQs

Reference

1. Trbojević-Akmačić, Irena, et al. "High-throughput glycomic methods." Chemical reviews 122.20 (2022): 15865-15913. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.1021/acs.chemrev.1c01031

Resources

• N-Glycosylation Analysis
• O-Glycosylation Analysis
• DNA Glycosylase and GlycoRNA Analysis
• Fc Region Glycosylation Analysis
• Specialized Glycoconjugate Analysis
• Glycosylation Inhibition

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